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  • Radiocarbon dating is a method for determining the age of an object containing organic C combines with...
  • Despite seeming like a relatively stable place, the Earth's surface has changed dramatically over the past 4.
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  • Carbon is a weakly radioactive isotope of Carbon; also known as radiocarbon ,...

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Loan on marksheet in bangalore dating Research Short note on radioactive carbon hookup method Adult archive Plant biomass consists primarily of carbohydrates derived from photosynthesis. Monitoring the assimilation of carbon via the Calvin-Benson cycle and its subsequent utilisation is fundamental to understanding plant growth. The use of stable and radioactive carbon isotopes, supplied to plants as CO 2 , allows the measurement of fluxes through the intermediates of primary photosynthetic metabolism, long-distance transport of sugars in the vasculature, and the synthesis of structural and storage components. Here we describe the design of a system for supplying isotopically labelled CO 2 to single leaves of Arabidopsis thaliana. We demonstrate that the system works well using short pulses of 14 CO 2 and that it can be used to produce robust qualitative and quantitative data about carbon export from source leaves to the sink tissues, such as the developing leaves and the roots. Time course experiments show the dynamics of carbon partitioning between storage as starch, local production of biomass, and export of carbon to sink tissues. This isotope labelling method is relatively simple to establish and inexpensive to perform.
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